PPG Home Page
Home page for the PPG program

 

DNASYSTEM
Web pages with Links
to other Bioinformatic Sites

 

UCSD NHLBI Program

Sympathetic Neuroeffector Junctions and Blood Pressure

Human Essential Hypertension

 

Biological Protocols

 

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The following is a list of biological or laboratory protocols or standard operating procedures (SOPs) used by the PPG program:

  1. Growth of PC12 cells
  2. poly-L-lysine coating of plates for PC12 secretion and transfections
  3. Harvesting PC12 cells for reporter assay (DTOC)
  4. Harvesting PC12 cells for reporter assay (LT)
  5. Luciferase assay protocol
  6. [3H]-catecholamine secretion study
  7. Transfection of PC12 cells (Lipofectamine)
  8. Transfection of PC12 cells (Superfect)
  9. Transfection of PC12 cells (Lipofectin)
  10. Transfection of PC12 cells (GenePorter)
  11. Metabolic labeling of cell proteins with [35S]-met
  12. Immunoprecipitation of metabolically labeled cells
  13. Immunoprecipitation for MALDI mass spectrometry
  14. 45Ca2+ cellular uptake
  15. CaPO4 Transfection for Chromaffin Cells
  16. Making antibodies to synthetic peptides
  17. Adrenal chromaffin granule (chromaffin vesicle) preparations
  18. Bovine chromaffin cell isolation
  19. 22Na+ uptake study in PC12 cells
  20. 45Ca++ uptake study in PC12 cells
  21. N-acetyl-beta-D-glucosaminidase activity (lysosomal enzyme marker)
  22. Human or bovine chromogranin A (hCgA or bCgA) RIA
  23. Catecholamine assay (Weil-Malherbe fluorometric assay method)
  24. Succinate dehydrogenase ("succinate INT") enzymatic activity (mitochondrial enzyme marker)
  25. Dopamine beta-hydroxylase (DBH) enzymatic activity assay
  26. Equilibrium radioligand binding of [125I]-catestatin to Torpedo nicotinic receptor-containing membranes.
  27. Secretoneurin (SN) radioligand binding assay.
  28. Simplified fluorometric assay of catecholamine.
  29. Urine Kallikrein Assay
  30. Nitrate/Nitrite Assay for urine, serum or saliva
  31. Buccal cell DNA isolation
  32. Genomic DNA isolation
  33. Amendment to Genomic DNA isolation protocol
  34. Genomic DNA "mouthwash" protocol
  35. Picogreen working solution - DNA quantitation
  36. Quantifying DNA using Fluoroskan Ascent and PicoGreen
  37. DNA Preparation for Quantification using PicoGreen
  38. Pharmacogenomics and Hypertension Draft
  39. Buffy coat DNA prep
  40. Preparation of a paraformaldehyde fixative solution
  41. Chemiluminescence detection of chimeric embryonic alkaline phosphatase (EAP) secretion from PC12 cells

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Latest modification: July, 2005

If you have comments or queries, or If you wish assistance in use of these files, please contact Doug Smith.